BETALAIST: EFFECTIVENESS OF BETA VULGARIS (BEETROOT) AS A SUBSTITUTE TO CRYSTAL VIOLET STAIN

Jhay Lord C. Mata

Valenzuela National High School

jhaylordmata28@gmail.com

2024

ABSTRACT

Scientists have extensively researched and utilized microscopic staining to apply stains to their material for experimental purposes. Microscopic stains are essential for experiments as they enhance the visibility and clarity of the resolution and structure of the object under the microscope. The researchers investigated the use of beetroot as a possible substitute for synthetic colors due to a growing shortage of these pigments. The main aim of this study was to assess the viability of using beetroot as a substitute for crystal violet stain, employing several statistical methods. The data was interpreted and analyzed using parametric and non-parametric test which are Chi-Square and T-Test Paired Two Samples for Means. The Chi-square test yielded scores of χ2=.9956 for crystal violet stain, χ2=1.95 for betalaist, and χ2=3.25 for beetroot. The T-Test Paired Two Sample for Means yielded a score of 0.09, which exceeds the significance level. According to the study's findings, beetroot has been identified as a potential substitute for crystal violet stain. As a result, we accept the null hypothesis. Based on the collected data, the researchers have determined that beetroot can be regarded as a highly promising substitute for microscopic stain. This is due to its outstanding visual performance which is comparable to that of crystal violet stain. Researchers proposed investigating the feasibility of utilizing beetroot for the production of various pigmented items, such as ink.

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